畜牧兽医学报2013，Vol.44Issue(3)：475-480,6.
白头翁素对腹泻小鼠肠黏膜修复因子EGFR和TGFβ1的影响
Effect of Anemonin on Intestinal Mucosa Repair Factors EGFR and TGFβ1 of Diarrheal Mice
摘要
Abstract
Anemonin is the main component of pulsatilla, it has conspicuous control efficiency on diarrhoea. The aim of this study was to clarify the intestinal mucosa repair mechanism of anemonin on diarrhoea. The experiment established the diarrhoea model by porcine rotavirus (PRV) and E. coli, immunohistochemistry and RT-PCR were used in the study. The results showed that: In the preliminary stage of diarrhoea, the protein of TGFβ1 and EGFR in self-healing group, treatment group and prevention group were all higher than that of control group, and the prevention group was significantly higher than that of control group (P<0. 05). The expression of EGFR mRNA and TGFβ1 mRNA in self-healing group, treatment group and prevention group were all significantly higher than that of control group (P<0. 01). In the later stage of diarrhoe-a, the expression of EGFR and TGFβ1 gradually decreased until to the normal condition. The results suggeste that the mechanism of anemonin could significantly prevent diarrhoea caused by PRV and E. coli maybe has some relationships with regulating the expression of EGFR and TGFβ1.关键词
白头翁素/小鼠/腹泻/黏膜修复/表皮生长因子受体/转化生长因子β1Key words
anemonin/ mice/ diarrhoea/ mucosa repair/ EGFR/ TGFβ1分类
农业科技引用本文复制引用
刘颖,徐倩倩,薛晓阳,褚军,肖静静,陈立功,董世山..白头翁素对腹泻小鼠肠黏膜修复因子EGFR和TGFβ1的影响[J].畜牧兽医学报,2013,44(3):475-480,6.基金项目
河北省科技支撑计划项目(07220401D-01) （07220401D-01）
