畜牧兽医学报2013，Vol.44Issue(3)：488-494,7.
东北地区野生鸟类J亚群禽白血病分子流行病学调查及部分基因组序列分析
Molecular Epidemiology and Part Genome Sequence Analysis of Subgroup J Avian Leukosis Virus Isolated from Wild Birds in Northeast China
摘要
Abstract
In order to analyze the situation of wild birds infected avian leukosis virus(ALV) in China in 2010-2011, we collected 300 samples of wild birds from different areas. Seven strains of ALV-J were detected by PCR. The env gene and the 3'UTR were amplified, cloned and se-quenced from seven isolated strains, which were subgroup J-positive. The results showed that the env genes of the seven strains from positive samples were not in the same branch with prototype strain HPRS-103 and representative strains, and the 3'UTR of them were a little different from HPRS-103. Part sites of E element have mutation, compared with HPRS-103. ALV has infected the wild birds in China. The large circumscription of the wild birds increases the difficulty of prevention and control of AL, though the relevance ratio is low. Parts of the env gene and 3'UTR of isolated strains have varied by sequencing and analyzing. More attention should be paid in preventing and controlling the transmission of ALV among wild birds.关键词
野生鸟类/禽白血病/流行病学调查/序列分析Key words
wild birds/ avian leukosis/ epidemiological survey/ sequence analysis分类
农业科技引用本文复制引用
李德龙,高玉龙,曾祥伟,杨波,刘婉思,高奇,秦立廷,高宏雷,王笑梅..东北地区野生鸟类J亚群禽白血病分子流行病学调查及部分基因组序列分析[J].畜牧兽医学报,2013,44(3):488-494,7.基金项目
公益性行业(农业)科研专项经费资助(201203055) （农业）
现代农业肉鸡产业技术体系建设(nycytx-42-G3-01) （nycytx-42-G3-01）
哈尔滨市科技攻关计划项目(2010AA6AN034) （2010AA6AN034）
中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室开放基金项目(SKLVBF201201) （SKLVBF201201）
