畜牧兽医学报2013，Vol.44Issue(3)：495-500,6.
渝西地区奶牛隐孢子虫的分离和基因型鉴定
Isolation and Genotype Identification of Cryptosporidium spp.in Dairy Calves in Western Chongqing
摘要
Abstract
To identify Cryptosporidium species and genotypes in Western Chongqing, 18S rRNA genes were amplified by nested PCR and the products were analyzed by RFLP. The amplified fragments were cloned into pEGM-T easy vector and sequenced. The homology and phylogeny analysis of these sequences were conducted by using BLAST and MEGA 4. 0 softwares. The results indicated that the approximate 830 bp fragments of 18S rRNA gene were successfully amplified, and the PCR product was digested with restriction enzymes Ssp Ⅰ and Mbo Ⅱ , respectively. All the digested products were analyzed with RFLP assay. The results revealed the presence of all the three major Cryptosporidium species of dairy calves viz. , C. andersoni, C. ryanae and C. bovis. C. andersoni is the dominant species in Western Chongqing. This work will provide a valuable resource for further research on pathogeny biology of cryptosporidiosis and monitor this disease in Chongqing.关键词
隐孢子虫/18S rRNA/PCR-RFLP/种系发育分析Key words
Cryptosporidium/ 18S rRNA/PCR-RFLP/phylogenetic analysis分类
农业科技引用本文复制引用
林洁,周荣琼,谭燕财,马光旭,敖冯虎,胡志刚..渝西地区奶牛隐孢子虫的分离和基因型鉴定[J].畜牧兽医学报,2013,44(3):495-500,6.基金项目
国家自然科学基金项目(31172313) （31172313）
重庆市自然科学基金项目(CSTC,2009BB113) （CSTC,2009BB113）
西南大学博士基金(11BSr04) （11BSr04）
