西南林业大学学报2013，Vol.33Issue(1)：39-44,6.DOI:10.3969/j.issn.2095-1914.2013.01.007
菊花EST-SSR分析及标记开发
EST-SSR Analysis and Marker Development for Chrysanthemum morifolium
摘要
Abstract
7087 EST of Chrysanthemum morifolium were assembled in order to provide molecular markers, and 275 contigs were obtained. There were 50 microsatellites (SSRs) were detected and averagely there was one SSR locus detected from 2 854. 3 bp of contigs. Trinucleotide repeats were the most abundant repeats (50. 00% ) a-mong these SSR types. As for the composition of microsatellites, AC, AG repeats were the richest motif in dinucle-otide repeats, and CAT, CCA repeats were the most frequent motifs in trinucleotide repeats, whereas (TTTN) n and (ATTTN ) n repeats were dominant in tetra- and penta-nucleotide repeats, respectively. All the dominant repeat motifs for different type of SSRs were rich in A and T alkali bases. In EST of C. morifolium, microsatellites longer than 20 bp accounted for about 00% of the detected SSRs. 428 pairs of primers were designed using Primer 5. 0 and Oligo 6. 0 according to these EST sequences containing SSR. 28 pairs of primers were randomly selected for PCR test with genomic DNA of Huangshan variety of Chrysanthemum morifolium, and 27 primer pairs succeeded in amplification, with successful ratio of 96. 4%.关键词
表达序列标签/简单重复序列/微卫星变异/菊花Key words
expression sequence tag (EST)/ simple sequence repeat (SSR)/ microsatellite variation/ Chrysanthemum morifolium分类
农业科技引用本文复制引用
万志兵,陈燕,闫莹莹,陈黎..菊花EST-SSR分析及标记开发[J].西南林业大学学报,2013,33(1):39-44,6.基金项目
安徽省教育厅自然科学项目(KJ2011B169)资助 （KJ2011B169）
高校省级优秀青年人才基金项目(2012SQRL185)资助. （2012SQRL185）
