烟草科技Issue(3)：77-82,6.
烤烟品种K326内生细菌分离、抗黑胫病菌株筛选及种群组成分析
Isolation, Screening and Phylogenetic Analysis of Antagonistic Endophytic Bacteria Against Phytophthora Parasitica var.Nicotianae from Flue-cured Tobacco Variety K326
摘要
Abstract
A total of 168 isolates with antagonistic effects against Phytophthora parasitica var. nicotianae (P. parasitica var.) were screened from 1000 endophytic bacteria isolated from flue-cured tobacco variety K326 at different growing stages, and bioassay results indicated that the inhibitory activities of these antagonists ranged from 12.54% to 50.14%. The biomass of endophytic bacteria was higher during the stages of seedling and rosette, which reduced by one order of magnitude during the stages of flowering and maturity; however, the number of antagonists kept an increasing trend. The RFLP analysis of 16S rRNA sequence on the 168 antagonists produced 10 RFLP patterns. Based on their RFLP patterns, 39 isolates were selected for 16S rRNA sequencing and phylogenetic analysis. The results showed that the 168 antagonists belonged to two groups of bacteria, namely Firmicutes and Actinobacteria, dominated by the genus Bacillus in Firmicutes group containing 6 RFLP patterns, 150 strains, including Bacillus amyloliquefaciens subsp. plantarum, Bacillus tequilensis, Bacillus methylotrophicus, Bacillus cereus, Bacillus thuringiensis and Bacillus aryabhattai. Bacillus amyloliquefaciens subsp. plantarum and Bacillus methylotrophicus emerged with the highest frequency. The remaining 18 strains belonged separately to 4 species, Brevibacillus formosus, Brevibacillus parabrevis, Pseudomonas umsongensis and Pseudomonas aeruginosa.关键词
烟草/K326/内生细菌/黑胫病Key words
Tobacco/ K326/ Endophytic bacterium/ Phytophthora parasitica var. nicotianae分类
农业科技引用本文复制引用
奚家勤,冯云利,薛超群,尹启生,莫明和,方敦煌,王广山,郭建华..烤烟品种K326内生细菌分离、抗黑胫病菌株筛选及种群组成分析[J].烟草科技,2013,(3):77-82,6.基金项目
郑州烟草研究院科技项目"烤烟抗病内生菌的分离及其应用研究"(122009CZ0420). （122009CZ0420）
