医药导报2013，Vol.32Issue(5)：561-564,4.DOI:10.3870/yydb.2013.05.003
姜黄素调控乳腺癌细胞增殖与转移机制研究
The Mechanism of Curcumin Inhibitting Breast Cell Proliferation and Migration
摘要
Abstract
Objective To investigate the influence of curcumin on hypoxia-inducible factor R1, α subunit ( HIF-1α) and cell proliferation and migration of the MDA-MB-453 cell line.Methods The effects of curcumin on MDA-MEM53 cell growth was studied by means of MTT and cell count assay, cell migration analysis was determined by a transwell assay.After cells were treated with curcumin under normoxia and hypoxia, the mRNA and protein levels of HIF-1α were measured by Real-time PCR and Western blot, while the mRNA and protein levels of vascular endothelial growth factor (VEGF) were measured by Realtime PCR and ELISA, respectively.Results Curcumin inhibited cell growth and migration of the MDA-MB-453 cell line.Being treated with curcumin, either normoxia or hypoxia, the mRNA level of HIF-1α was unchange, while the protein level of HIF-1α was significantly decreased.Both the mRNA and protein levels of VEGF were obviously down-regulated.Conclusion Curcumin regulates the proliferation and migration of MDA-MB-453 cells through HIF-1α.关键词
姜黄素/乳腺癌/缺氧诱导因子-1α/细胞增殖/细胞转移Key words
Curcumin/Breast carcinoma/HIF-1α/Proliferation/Migration分类
医药卫生引用本文复制引用
蔡少鑫,陈成,杨熹,李小兰,李兆明,胡俊波..姜黄素调控乳腺癌细胞增殖与转移机制研究[J].医药导报,2013,32(5):561-564,4.基金项目
国家973计划资助项目(2009CB521802) （2009CB521802）
国家自然科学基金资助项目(30872472,30973496,30800569) （30872472,30973496,30800569）
