医药导报2013，Vol.32Issue(5)：583-585,3.DOI:10.3870/yydb.2013.05.009
大黄素对结肠癌模型鼠两种细胞因子影响
Influence of Emodin on VEGF-C and MMP-9 of Mice with Colon Cancer
摘要
Abstract
Objective To explore antitumor effeet of emodin on nude mice with human colon cancer xenografts, and VEGF-C and MMP-9 expression.Methods The human colon cancer xenografts model in nude mice were established and the mice were randomly divided into 5 groups with 10 mice in each.The emodin group were injected daily with emodin at doses of 50, 100 and 150 mg·kg-1 , and the positive control group was injected daily with 5-Fu at the dose of 20 mg·mg-1 while the positive control group was injected daily with normal saline.The tumor inhibition rate was observed and apoptosis index was detected by TUNEL, the expression of matrix metalloproteinase (MMP)-9 and vascular endothelial growth factor (VEGF)-C were investigated by immunohistochemical analysis.Results Compared with the model group, the tumor volume, tumor mass, tumor inhibition rate and apoptosis index of each dose group were significantly decreased (P<0.05 , P<0.01), and the expression of VEGF-C and MMP-9 was lowered at various degree.VEGF-C expression in the medium and high dose of emodin group was significantly weaker(0.68±0.36) ,(0.22±0.41) than that in the model group(2.11 ±0.45 ) (P<0.05, P<0.01) and MMP-9 expression(l.11 ±0.41) , (0.38±0.44) was significantly lower than that in the model group(2.31 ±0.39) (P<0.05, P<0.01).Conclusion Emodin effectively inhibits tumor growth, the mechanism of which may be related to the induction of apoptosis and inhibition of VEGF-C and MMP-9 expression.关键词
大黄素/结肠癌移植瘤/凋亡指数/基质金属蛋白酶9/血管内皮生长因子CKey words
Emodin/Human colon cancer xenografts/ Apoptosis index/Matrix metalloproteinase-9/ Vascular endothelial growth factor-C分类
医药卫生引用本文复制引用
张延新,宋文刚,高风兰..大黄素对结肠癌模型鼠两种细胞因子影响[J].医药导报,2013,32(5):583-585,3.基金项目
漯河医学高等专科学校2012年自然科学研究资助计划项目(2010S06) （2010S06）
