中国临床药理学杂志2013，Vol.29Issue(3)：199-201,3.
华蟾素注射液对与人淋巴管内皮细胞共培养下人肝癌HepG-2细胞的增殖及侵袭的影响
Effect of Cinobufacini Injection on proliferation and invasiveness of human hepatoma HepG-2 cells co-cultured with human lymphatic endothelial cells
摘要
Abstract
Objective To study the effect of Cinobufacini Injection on proliferation, heterogeneous adhesion and invasiveness of human hepatoma HepG - 2 cells co - cultured with human lymphatic endothelial cells (HLEC). Methods Co - culture system of human hepatoma HepG - 2 cells and HLEC was established by means of Transwell chamber. Cell proliferation was analysed by Trypan blue stain assay. MTT assay was used to observe the heterogeneous adhesion capacity of HepG - 2 cells co - cultured with HLEC. Transwell invasion chamber was used to observe the invasiveness capacity of HepG - 2 cells co - cultured with HLEC. Results Cinobufacini Injection significantly inhibits proliferation, heterogeneous adhesion and invasiveness of hepG - 2 cells co - cultured with HLEC in dose dependent ways( all P <0. 05). Conclusion Cinobufacini Injection can inhibit the capability of proliferation, invasiveness and heterogeneous adhesion of HepG - 2 cells, wich might contribute the inhibiting mechanisms of Cinobufacini Injection on tumor metastasis.关键词
华蟾素注射液/HepG-2细胞/人淋巴管内皮细胞/增殖/异质粘附/侵袭Key words
Cinobufacini Injection/hepG - 2 cell/human lymphatic endothelial cells/proliferation/heterogeneous adhesion/invasiveness分类
医药卫生引用本文复制引用
付海燕,高山,田莉莉,陈秀英,崔晓楠..华蟾素注射液对与人淋巴管内皮细胞共培养下人肝癌HepG-2细胞的增殖及侵袭的影响[J].中国临床药理学杂志,2013,29(3):199-201,3.基金项目
国家自然科学基金资助项目(81173615) （81173615）
