Abstract
Objective To investigate the correlation between angiopoietin -2 ( Ang -2 ) A1087G polymorphism and type 2 diabetic nephropathy ( DN ). Methods ( 1 ) A total of 1 021 patients with type 2 diabetes mellitus ( T2DM group ) and 254 healthy people ( NC group ) visiting the First People's Hospital of Yunnan Province and the Second Affiliated Hospital of Kunming Medical University between January 2010 and January 2012 were selected. According to urinary albumin excretion rate ( UAER ), the T2DM group was divided into three subgroups: T2DM without DN group ( DN0 group, 431 cases ), T2DM with microalbuminuria group ( DN1 Group, 322 cases ), and T2DM with massive proteinuria group ( DN2 group, 268 cases ). Clinical and biochemical indicators of subjects in each group were collected. The exon 4 of Ang -2 A1087G was tested for polymorphism. Results ( 1 ) Ang -2 A1087G gene frequency and allele frequency of subjects in each group were significantly different ( P < 0. 05 ). The DN0 , DN1, and DN2 group's AG + GG frequency ( 39. 0% , 41. 9% and 44. 8% ) and the G allele frequency ( 37. 8% , 30. 3% and 33. 6% ) were significantly higher than those of the NC group ( 24. 4% and 19. 1% , P <0. 05 ).( 2 ) Significant differences were observed among the groups in blood pressure, glycosylated hemoglobin ( HbA1c ), glomerular filtration rate ( eGFR ), fasting blood glucose ( FBG ), fasting insulin ( FINS ), β - cell function index ( HOMA - IS ), insulin resistance index ( HOMA - IR ), monocyte chemoattractant protein - 1 ( MCP - 1 ), and Ang - 2 level ( P < 0. 05 ). ( 3 ) Multiple Logistic regression analysis showed that Ang -2 A1087G AG + GG genotype, G allele, serum Ang -2 level, systolic blood pressure, HbA1c, FBG, MCP - 1, HOMA - IS, and eGFR had correlation with DN ( P < 0. 05 ). After adjusting for age, sex, disease course, HbA1c, blood pressure, and other factors, AG + GG genotype had 2. 823 times the risk of developing DNO [ 95% CI ( 1. 557 , 4.221), P=0.011], 2. 778 times the risk of DN1 [ 95% CI ( 2. 356, 5.220), P=0.010], and 2. 991 times the risk of DN2 [ 95% CI ( 3. 560, 6. 229 ), P = 0. 001 ] compared with AA genotype. G allele had 1. 442 times the risk of developing DNO [ r = 1. 321, OR = 1.442, 95% CI ( 2. 320, 4. 178 ), P = 0. 030 ], 1. 984 times the risk of DNl[r = 1.021, OR = 1.984, 95% CI (2. 621, 4.574), P =0.027 ], 1. 881 times the risk of DN2 [ r = 1. 338, OR = 1. 881, 95% CI ( 3. 321 , 5. 173 ), P =0. 001 ] compared with that for allele A. Conclusion Ang -2 A1087G gene polymorphism was associated with the incidence of type 2 diabetic nephropathy. G allele may be genetic risk factors for the development of diabetic nephropathy.关键词
血管生成素2/多态性,单核苷酸/糖尿病,2型/糖尿病肾病Key words
Angiopoietin - 2/ Polymorphism, single nucleotide/ Diabetes mellitus, type 2/ Diabetic nephropathy分类
医药卫生
