中国比较医学杂志2013，Vol.23Issue(3)：17-20,后插2,5.DOI:10.3969.j.issn.1671.7856.2013.003.005
DEN诱发PLCε基因敲除小鼠肝癌模型的建立
Establishment of DEN induced PLCε knock-out mouse liver cancer model
摘要
Abstract
Objective To establish animal models of liver tumors, the PLCe gene knockout mice was induced by mutation inducers Diethylnitrosamine ( dielhylnitrosamine DEN ) combined with tumor enhancer phenobarbital (phenobarbital PB). Methods 12-day-old male PLCε-/- and PLCε +/+ mice were randomly selected as group Ⅰ (n = 40) and group Ⅱ (n=40) ,respectively. The mice of groupⅠand Ⅱwere injected DEN through abdominal cavity at the age of 12 days, then drinking PB after 4 weeks and continuously until 24 weeks. At the same time, 12-day-old male PLCε-/-and PLCε+/+ mice were randomly selected as the control group Ⅰ ( n =40) and control group Ⅱ ( n =40) , respectively, and fed with normal feeds for 24 weeks. At the end of the experiment, the liver tumor was observed under electron microscopy. Results Pathology results confirm that 18 mice of group Ⅱ had liver tumors, and rate of tumor formation is 60% ; 15 mice of group Ⅰ had liver tumors, and rate of tumor formation is 46. 9% ; the mice of control group Ⅰ and Ⅱ had not liver tumors. Conclusions The animal model of liver tumor have been successfully established by using mutation inducers DEN combined with PB in PLCe gene knockout mice, and could provide a foundation for further researching effects of PLCs during liver tumor formation.关键词
肝脏肿瘤/基因敲除/PLCε/动物模型Key words
Liver tumors/Gene knockout/PLCε/Animal models分类
医药卫生引用本文复制引用
崔智,李瑞生,李晓娟,胡燕,戴广海,白云峰..DEN诱发PLCε基因敲除小鼠肝癌模型的建立[J].中国比较医学杂志,2013,23(3):17-20,后插2,5.基金项目
北京市自然基金面上项目(7102144). （7102144）
