中国畜牧兽医2013，Vol.40Issue(3)：54-58,5.
鲤鱼γ-2β干扰素基因组DNA克隆及序列分析
Cloning and Sequence Analysis of Interferon γ-2β (IFN γ-2β) Genomic DNA in Common Carp (Cyprinus carpio L.)
摘要
Abstract
The research aimed to carry out the cloning, identification and sequence analysis of genomic DNA of carp interferon γ-2β (IFNγ-2β). On the basis of the full-length cDNA of carp, we designed two pairs of primers in its bilateral untranslated regions and the exons to perform PCR that used the carp spleen genomic DNA as the template, the IFNγ-2β full-length genomic DNA sequence was obtained. The full-length genomic DNA was 2084 bp, the GenBank accession number was JX181981, the gene sequence analysis showed the evolution of conservative and contained 4 exons and 3 introns were like most of the other species, the number of bases of each exon closed to the mammalian exons correspondingly. The exon and intron splice sites were complied with GT-AG rules.关键词
鲤鱼/干扰素γ-2β/克隆/序列分析Key words
common carp/ interferon γ-2β/ cloning/ sequence analysis分类
生物科学引用本文复制引用
陈义龙,孙真,贾生美,冯祥汝,王文东,张俊辉,杨振国,卢强..鲤鱼γ-2β干扰素基因组DNA克隆及序列分析[J].中国畜牧兽医,2013,40(3):54-58,5.基金项目
国家自然科学基金项目(30972277) （30972277）
吉林大学基本科研业务费项目(200903250). （200903250）
