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不同样品中犬输血传播性病毒的PCR检测比较与基因分析

郁达义 叶剑波 袁聪俐 华修国

中国畜牧兽医2013,Vol.40Issue(3):59-62,4.
中国畜牧兽医2013,Vol.40Issue(3):59-62,4.

不同样品中犬输血传播性病毒的PCR检测比较与基因分析

The PCR Detection and Gene Analysis of Torque-Teno Canis Virus in Different Extraction Samples

郁达义 1叶剑波 1袁聪俐 1华修国1

作者信息

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摘要

Abstract

Based on the sequence of Torque-Teno canis virus registered on NCBI (Cf-TTV10, accession No. AB076002), we designed primers for nested PCR.to detect the Torque-Teno canis virus in serum and fecal samples. It was showed that there were 14. 6% Torque-Teno canis virus positive canine serum samples and 12. 7% positive fecal samples, and it was verified that the gene type was same. There was no significant difference in different extraction samples (P>0. 05). This research provided the basis for the further study of TTV characteristics and molecular epidemiology.

关键词

犬输血传播性病毒/PCR检测/基因分析

Key words

Torque-Teno canis virus/PCR detection/gene analysis

分类

农业科技

引用本文复制引用

郁达义,叶剑波,袁聪俐,华修国..不同样品中犬输血传播性病毒的PCR检测比较与基因分析[J].中国畜牧兽医,2013,40(3):59-62,4.

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