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免疫组化法检测促黄体素受体在多浪羊和卡拉库尔羊卵巢上的分布

王惠娥 汪澜 盛伟明

中国畜牧兽医2013,Vol.40Issue(3):126-129,4.
中国畜牧兽医2013,Vol.40Issue(3):126-129,4.

免疫组化法检测促黄体素受体在多浪羊和卡拉库尔羊卵巢上的分布

Distribution and Localization of Luteinizing-hormone Receptors on the Ovaries between Duolang Sheep and Karakul Sheep

王惠娥 1汪澜 1盛伟明1

作者信息

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摘要

Abstract

In order to understand the endocrine regulation process in a morphological view and reveal luteotropic hormone (LH) mechanism action in the female mammalian ovary, the distribution and localization of luteotropic hormone receptors (LHR) in ovaries of Duolang sheep and Karakul sheep were determined by immunohistochemical SP method. The results showed that 5 positive continuous sections were selected respectively. LHR-immunoreactive substance was mainly distributed over granulose cells, theca cells, oocyte, and ovary blood vessel surrounding interstitium. LHR was mainly loaded by the gran-ulose cells and theca cells. Receptors regulation was showed during the earlier follicle period. With the development of follicles, the quantities and the staining intensity of the LHR-immunoreactive cells presented an increasing trend.

关键词

多浪羊/卡拉库尔羊/促黄体生成素/促黄体生成素受体/免疫组织化学SP染色法

Key words

Duolang sheep/ Karakul sheep/ luteotropic hormone/ luteotropic hormone receptors/ immunohistochemical SP method

分类

生物科学

引用本文复制引用

王惠娥,汪澜,盛伟明..免疫组化法检测促黄体素受体在多浪羊和卡拉库尔羊卵巢上的分布[J].中国畜牧兽医,2013,40(3):126-129,4.

基金项目

兵团青年创新资金专项(2011CB001) (2011CB001)

校长基金(TDZKSS201106). (TDZKSS201106)

中国畜牧兽医

OA北大核心CSTPCD

1671-7236

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