中国畜牧兽医2013，Vol.40Issue(3)：130-133,4.
雌激素对奶牛输卵管上皮细胞前列腺素E2和F2α合成酶mRNA表达的影响
Effect of Estrogens on the Expression of PGES and PGFS mRNA in Bovine Oviduactal Epithelial Cells
摘要
Abstract
In order to explore the expression of prostaglandin E2 and F2α synthase (PGES and PGFS) mRNA in bovine ovi-ductal epithelial cells by estrogen, investigation the function of physiology in bovine. We used bovine regenerating epithelial cells in vitro training system, different times(0, 2 , 4, 8,16, 24 and 48 h) and different concentrations of estrogen (0,10-12, 10-11,10-10 and 10-9 mol/L) were added, and measured PGES and PGFS mRNA expression by using Real-time PCR. The results showed that different concentrations of E2 or different stimulation time in the same concentration of E2 could increase the expression of prostaglandin E2 synthase (PGES), but in addition of E2 4 h and the concentration was 10-10 mol/L compared with the control was extremly significant different (P<0. 01), while PGFS in addition of E2 24 h and the concentration was 10-12 mol/L compared with the control was extremly significant different (P<0. 01). Experimental results showed that estrogen could promote the expression of PGES and PGFS mRNA in oviduct epithelial cells of bovine, and regulate the mRNA expression of prostaglandin synthase PGES and PGFS.关键词
雌激素/输卵管上皮细胞/PGES/PGFS/荧光定量PCRKey words
estrogen/oviductal epithelial cells/PGES/PGFS/Real time RT-PCR分类
生物科学引用本文复制引用
樊凤娇,董至恒,付改玲,黄娜,曹金山..雌激素对奶牛输卵管上皮细胞前列腺素E2和F2α合成酶mRNA表达的影响[J].中国畜牧兽医,2013,40(3):130-133,4.基金项目
教育部留学回国人员科研启动基金(201-204015) （201-204015）
内蒙古自治区高等学校科学研究重点项目(NJ10049) （NJ10049）
国家自然科学基金(31160490). （31160490）
