中国畜牧兽医2013，Vol.40Issue(3)：146-149,4.
土槿皮乙酸B通过活性氧和有丝分裂原蛋白激酶诱导MCF-7细胞凋亡的研究
Study of Pseudolaric Acid B Induced Apoptosis through ROS Regulating MAPK in MCF-7 Cells
摘要
Abstract
The assay was aimed to study the relationship of ROS and MAPK family protein in pseudolaric acid B (PAB)-in-duced MCF-7 cell apoptosis. Contrast phase microscope was used to observe cellular morphologic alternation; MTT analysis detected the inhibitory ratio; flow cytometric analysis of DCFH-DA staining determined the level of ROS, and Western blotting detected the protein expression. We found that PAB induced MCF-7 cell apoptosis through increasing ROS and increased the phosphoration of JNK and decrease the phosphoration of ERK.关键词
土槿皮乙酸B/活性氧/凋亡/MAPK家族蛋白/人乳腺癌MCF-7细胞Key words
pseudolaric acid B/ROS/apoptosis/MAPK/MCF-7 family protein分类
生物科学引用本文复制引用
刘春禹,郑桂彬,于静华..土槿皮乙酸B通过活性氧和有丝分裂原蛋白激酶诱导MCF-7细胞凋亡的研究[J].中国畜牧兽医,2013,40(3):146-149,4.基金项目
吉林省中医药管理局(2010-pt064) （2010-pt064）
吉林省中医药管理局(2012-030). （2012-030）
