中国药房2013，Vol.24Issue(11)：964-967,4.DOI:10.6039/j.issn.1001-0408.2013.11.02
雷公藤甲素诱导肝细胞L-02凋亡的作用机制研究
Mechanism Research of Human L-02 Cells Apoptosis Induced with Triptolide
摘要
Abstract
OBJECTIVE: To study the mechanism of triptolide-induced L-02 cells apoptosis. METHODS: L-02 cells were cultured in vitro. The inhibitory effect of triptolide on the proliferation of L-02 cells was evaluated using MTT assay. Hoechst 33258 fluorescent staining was used to observe themorphological changes of apoptotic cells. Flow cytometry was performed to determine the apoptotic rate. The protein expression of p53, Bcl-2, Bax, Cyt-c in mitochondria and cytosol were detected using Western blot analysis. RESULTS: L-02 cells are treated with different concentrations of triptolide (60, 120 and 180 nmol/L) and same concentration of triptolide for different times (12, 24 and 48 h). It showed that inhibition effect of triptolide on cell proliferation was positively correlated with the concentration of triptolide or the time. Hoechst 33258 staining revealed typical apoptotic morphological changes. Western blot analysis suggested that the contents of p53, Bax and cytosol Cyt-c enhanced significantly (P<0.05) , and Bcl-2 and the content of Cyt-c in mitochondria weakened significantly (P<0.05) after treatment with triptolide 120 nmol/L. CONCLUSION: Triptolide probably may induce the apoptosis of L-02 cells by up-regulating proteins expression of Cyt-c in p53, Bax and cytosol and down-regulating proteins expression of Cyt-c in Bcl-2 and mitochondria.关键词
雷公藤甲素/人肝细胞L-02/凋亡Key words
Triptolide/ Human liver cell L-02/ Apoptosis分类
医药卫生引用本文复制引用
姚金成,刘颖,胡领,张陆勇,饶健,曾令贵,江振洲,何玲..雷公藤甲素诱导肝细胞L-02凋亡的作用机制研究[J].中国药房,2013,24(11):964-967,4.基金项目
财政部行业科研专项资助课题(No.200707008) （No.200707008）
湖南省科技厅科技计划项目(No.2009FJ3027). （No.2009FJ3027）
