中国药房2013，Vol.24Issue(11)：979-982,4.DOI:10.6039/j.issn.1001-0408.2013.11.07
菟丝子黄酮对脑缺血再灌注损伤模型大鼠脑组织中炎症反应的影响
Effects of Cuscutae Semen Flavonoids on Inflammatory Response of Brain Tissue in Rats with Cerebral Ischemia-reperfusion Injury
摘要
Abstract
OBJECTIVE: To study the effects of Cuscutae Semen flavonoids on inflammatory response of brain tissue in rats with cerebral ischemia-reperfusion injury. METHODS: Cerebral ischemia-reperfusion injury model of middle cerebral artery occlusion was induced by thread occlusion method of internal carotid. Model rats were divided into sham operation group (constant volume of normal saline), model group (constant volume of normal saline), Nimodipine group (12 mg/kg) group and Cuscutae Semen flavonoids high-dose and low-dose groups (100, 50 mg/kg). Neurological deficits score was conducted at the end of reperfu-sion; immunohistochemical staining and Western Blotting assay were used to determine the expression of TNF-α , IL-1β and ICAM-1 in brain tissue of rats. RESULTS: Compared with sham operation group, neurological deficits of rats were improved significantly in model group, and the expressions of TNF-α, IL-1β and ICAM-1 in cerebral tissue increased significantly (P<0.01); compared with model group, neurological deficits of rats were improved significantly in Cuscutae Semen flavonoids high-dose and low-dose groups, while the expressions of TNF-α, IL-1β and ICAM-1 decreased significantly(P<0.01). CONCLUSION: Cuscutae Semen flavonoids have a protective effect on cerebral ischemia-reperfusion injury, and its mechanism may be related to inhibition of inflammatory cytokine secretion and expression and reduce the inflammation of brain tissue.关键词
菟丝子黄酮/缺血再灌注损伤/肿瘤坏死因子-α/白介素-1β/细胞间黏附分子-1Key words
Cuscutae Semen flavonoids/ Cerebral ischemia-reperfusion injury/ TNF-α/ IL-1β/ ICAM-1分类
医药卫生引用本文复制引用
杨迪,王桂敏,翟宏颖..菟丝子黄酮对脑缺血再灌注损伤模型大鼠脑组织中炎症反应的影响[J].中国药房,2013,24(11):979-982,4.
