中国药房2013，Vol.24Issue(11)：1028-1030,3.DOI:10.6039/j.issn.1001-0408.2013.11.25
分光光度法测定巴豆油聚乳酸羟基乙酸微球中总油的含量
Determination of Total Oil Content in Croton Oil PLGA Microspheres by Spectrophotometry
摘要
Abstract
OBJECTIVE: To study a method for the determination of total oil content in Croton oil(poly-lactic-co-glycolic acid, PLGA) microspheres. METHODS: Croton oil microspheres broken by lye-ultrasonic, and main ingredient linoleic acid turned into conjugated linoleic acid through isomerization reaction. UV spectrophotometry was used to determine the absorbance, and total oil content of Croton oil microspheres was calculated by substituting conversion factor (ratio of unit concentration of conjugated linoleic acid to absorbance of croton oil) into standard curve equation. RESULTS: The linear range of conjugated linoleic acid was 1.571-9.427 μg/ml(r=0.999 9) with average recovery of 100.45% (RSD=0.60% ,n=6); conversion factor K was equal to 2.201 ± 0.043. CONCLUSION: The method is accurate and simple, and it can be used for the determination of total oil content in Croton oil PLGA microspheres.关键词
巴豆油/微球/分光光度法/异构化/共轭亚油酸/含量测定Key words
Croton oil/ Microspheres/ Spectophotometry/ Isomerization/ Conjugated linoleic acid/ Content determination分类
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李云霄,黄宝群,孙洪胜..分光光度法测定巴豆油聚乳酸羟基乙酸微球中总油的含量[J].中国药房,2013,24(11):1028-1030,3.
