中国药房2013，Vol.24Issue(12)：1132-1134,3.DOI:10.6039/j.issn.1001-0408.2013.12.28
HPLC法测定丁酸氢化可的松凝胶中丁酸氢化可的松的含量
Content Determination of Hydrocortisone Butyrate in Hydrocortisone Butyrate Gel by HPLC
摘要
Abstract
OBJECTIVE: To develop a method for the content determination of hydrocortisone butyrate in Hydrocortisone butyrate gel. METHODS: HPLC method was adopted. The determination was performed on Phenomen C18 column with mobile phase consisted of acetonitrile-0.5% acetic acid (51:49) at flow rate of 1.0 ml/min. The column temperature was 25 ℃ and injection volume was 20 μl. The sensitivity of the sample was 1.0 AUFS. RESULTS: The linear range of hydrocortisone butyrate was 97.6-878.4 ng (r=0.999 1) with an average recovery of 99.5% (RSD=0.45% , n=9). CONCLUSION: The method is proven to be simple, accurate and precise for the quality control of Hydrocortisone butyrate gel.关键词
丁酸氢化可的松凝胶/丁酸氢化可的松/高效液相色谱法/含量测定Key words
Hydrocortisone butyrate gel/ Hydrocortisone butyrate/ HPLC/ Content determination分类
医药卫生引用本文复制引用
王秋桐,王伟,侯海玲,王玉华..HPLC法测定丁酸氢化可的松凝胶中丁酸氢化可的松的含量[J].中国药房,2013,24(12):1132-1134,3.
