中国医科大学学报2013，Vol.42Issue(1)：19-22,4.
14-3-3蛋白调节1-细胞期小鼠受精卵有丝分裂
Regulation of 14-3-3 in the First Mitotic Cell Cycle in One-cell Stage Mouse Fertilized Eggs
摘要
Abstract
Objective To study the effects of 14-3-3 proteins in regulation of the first mitotic cell cycle in one-cell stage mouse fertilized eggs. Methods 14-3-3 isoform in the mouse fertilized eggs was identified by RT-PCR. 14-3-3 siRNA was introduced to G1 phase fertilized eggs by microinjection to study the cleavage rate, morphology and MPF activity. Results 14-3-3 ε was identified in one-cell stage of mouse fertilized eggs. Compared with the control group,the cleavage rate in pSUPER-14-3-3ε siRNA injection group was significantly decreased, mitosis was delayed and more abnormal morphology eggs were observed. Moreover, the maximal value of MPF activity was significantly decreased. Conclusion 14-3-3 proteins play critical roles in the first mitotic cell cycle in mouse fertilized eggs.关键词
14-3-3蛋白/1-细胞期受精卵/细胞周期Key words
14-3-3 protein/ one-cell stage fertilized egg/ cell cycle分类
医药卫生引用本文复制引用
崔城,秦鑫,任秀丽,于秉治..14-3-3蛋白调节1-细胞期小鼠受精卵有丝分裂[J].中国医科大学学报,2013,42(1):19-22,4.基金项目
国家自然科学基金资助项目(30900514) （30900514）
辽宁省教育厅高校科研计划(2008S224) （2008S224）
