中国医科大学学报2013，Vol.42Issue(1)：42-44,48,4.
单核细胞增生性李斯特菌fbpa基因敲除菌株的构建
Construction of fbpA-deletion Mutant of Listeria Monocytogenes
摘要
Abstract
Objective To construct an fbpA-deletion mutant of Listeria monocytogenes. Methods The fbpA gene and its upstream, downstream genes of Listeria monocytogenes were cloned into plasmid pCR Ⅱ. The upstream and downstream fragments were ligated into the pAULA using restriction enzyme as pAULA-ΔfbpA. To achieve allelic exchange, pAULA-ΔfbpA was introduced into Listeria monocytogenes by electroporation. The mutant was confirmed by PCR and Western blot. Results The fbpA gene was not detected in genome of fbpA-deletion mutant of Listeria monocytogenes,and FbpA was not expressed in fbpA-deletion mutant of Listeria monocytogenes. Conclusion The fbpA-deletion mutant of Listeria monocytogenes was constructed successfully.关键词
单核细胞增生性李斯特菌/fbpa/基因敲除Key words
Listeria monocytogenes/ fbpa/ gene knockout分类
医药卫生引用本文复制引用
李胜军,阎雪晶,王舰..单核细胞增生性李斯特菌fbpa基因敲除菌株的构建[J].中国医科大学学报,2013,42(1):42-44,48,4.基金项目
辽宁省教育厅高校科研计划(2008799) （2008799）
