中国医科大学学报2013，Vol.42Issue(1)：56-59,64,5.
白藜芦醇增加脂肪细胞3T3-L1中脂联素表达的作用机制探讨
Mechanisms of Resveratrol-mediated Increasing of Adiponectin Expression Increased in 3T3-L1 Adipocytes
摘要
Abstract
Objective To investigate the mechanism by which resveratrol enhances adiponectin expression in 3T3-L1 cells. Methods 3T3-L1 cells were treated with vehicle, resveratrol, TNF-α, or combination of resveratrol and TNF-α; released adiponectin was measured by ELBA; adiponectin and PPAR-γ mRNA levels were analyzed using real time PCR;PPAR-γ activity was determined with PPAR-γ activity kit. Results Compared with vehicle-treated control, PPAR-γ and adiponectin expressions were enhanced and suppressed in differentiated 3T3-L1 cells treated with resveratrol and TNF-α, respectively. Compared with TNF-a alone treatment, combined treatment with resveratrol recovered TNF-α mediated suppression of adiponectin expression and release; resveratrol compromised the suppressive effect of TNF-α on mRNA expression and activity of PPAR-γ (P< 0.05). Conclusion Resveratrol recovered TNF-a mediated reduction of adiponectin expression and release via regulation of PPAR-γ activity.关键词
白藜芦醇/肿瘤坏死因子/脂联素/过氧化物酶体增生物激活受体γ/脂肪细胞Key words
resveratrol/ tumor necrosis factor α/ adiponectin/ PPAR-γ/ adipocyte分类
医药卫生引用本文复制引用
王添,都镇先,张海燕,邓娓娓..白藜芦醇增加脂肪细胞3T3-L1中脂联素表达的作用机制探讨[J].中国医科大学学报,2013,42(1):56-59,64,5.基金项目
辽宁省科技攻关项目(2009225012) （2009225012）
