中国医科大学学报2013，Vol.42Issue(1)：65-68,4.
17-AAG对MCF-7细胞增殖的抑制作用及对STAT3和VEGF表达的影响
17-AAG Inhibits Proliferation of MCF-7 Human Breast Cancer Cells and Down-regulates VEGF and STAT3 Expressions
摘要
Abstract
Objective To investigate the effects of 17-AAG on VEGF and STAT3 expressions in human breast cancer MCF-7 cells. Methods MCF-7 cells were cultured in DMEM medium; MTT method was performed to evaluate the inhibitory effects of 17-AAG with different time and different doses on proliferation of MCF-7 cells;the genes and proteins expressions of VEGF and STAT3 were determined by RT-PCR and Western blot analysis. Results With treatment of 0.165-10.000 mg/L 17-AAG for 24 hours and 48 hours, significantly inhibition of the MCF-7 cells proliferation was observed in both time- and dose-dependent manner. The assay of RT-PCR and Western blot showed that the genes and proteins expressions of VEGF and STAT3 in MCF-7 cells induced by 17-AAG were significantly down-regulated in a dose-dependent manner when the cells were treated with 1.0,2.0,3.0,5.0 mg/L 17-AAG for 48 hours. Conclusion 17-AAG can inhibit the cell proliferation and down regulate the genes and proteins expressions of VEGF and STAT3 of human breast cancer MCF-7 cells in vitro.关键词
17-AAG/人乳腺癌MCF-7细胞/细胞增殖/VEGF/STAT3Key words
17-AAG/ breast cancer MCF-7 cell/ proliferation/ VEGF/ STAT3分类
医药卫生引用本文复制引用
肖丽君,赵恩宏,赵爽,高艺铭,郭璐,许倩..17-AAG对MCF-7细胞增殖的抑制作用及对STAT3和VEGF表达的影响[J].中国医科大学学报,2013,42(1):65-68,4.基金项目
河北省科技厅科学技术项目(072761450) （072761450）
河北省教育厅自然科学项目(2007326) （2007326）
河北省人口和计划生育委员会科技项目(2011-A15) （2011-A15）
