中国药理学通报2013，Vol.29Issue(5)：622-625,4.DOI:10.3969/j.issn.1001-1978.2013.05.008
表没食子儿茶素没食子酸酯抗甲型流感病毒的作用研究
Anti-influenza A virus activity of epigallocatechin gallate
摘要
Abstract
Aim To explore the anti-influenza A virus activity of epigallocatechin gallate ( EGCG ) and its mechanism. Methods Inhibition of viral-entry by EGCG was determined with luciferase assay in MDCK cells infected with A/Thailand/Kan353/2004 H5N1 pseudovirus. Nneuraminidase inhibition assay was further used to investigate the mechanism of anti-influenza activity. Anti-influenza activity of EGCG was further confirmed in MDCK cells infected with influenza A virus FM_1 strain with different medications, including treated, prophylactic medications. Results EGCG inhibited the entry of H5N1 pseudovirus into MDCK cells with IC50 of 145. 10 μmol · L-1. EGCG also inhibited the activity of neuraminidase with IC50 of 417. 20 μmol · L-1. EGCG also showed anti-influenza viral effect on FM_1 virus in MDCK cells. IC50 was 6. 88 μmol · L-1 when added before inoculation with influenza A virus FM_1 strain, while IC50 was 14. 83 μmol · L-1 when added after the virus inoculation. TI was 58. 02 and 26. 92, respectively. Conclusions EGCG can inhibit influenza A virus in vitro. The mechanism of EGCG includes at least the virus entry and neuraminidase activity inhibition.关键词
表没食子儿茶素没食子酸酯/甲型流感病毒/H5N1亚型/H1N1亚型/血凝素/神经氨酸酶Key words
EGCG/ influenza A virus/ H5N1 subtype/ H1N1 subtype/ hemagglutinin/ neuraminidase分类
医药卫生引用本文复制引用
李湘潋,刘叔文,杨洁..表没食子儿茶素没食子酸酯抗甲型流感病毒的作用研究[J].中国药理学通报,2013,29(5):622-625,4.基金项目
国家自然科学基金资助项目(No 81102792) （No 81102792）
广东高校国际科技合作创新平台项目(No gjhz1105) （No gjhz1105）
