中国药理学通报2013，Vol.29Issue(5)：632-637,6.DOI:10.3969/j.issn.1001-1978.2013.05.010
基于代谢组学的六味地黄丸干预大鼠甲亢模型的作用研究
Serum metabonomic study on liuwei dihuang pills in the treatment of hyperthyroidism
摘要
Abstract
Aim To explore the underlying mechanism of hyperthyroidism induced by subcutaneous injection of L-levothyroxine sodium and and intervention effects of Liuwei Dihuang pills on sprague dawley rats via serum metabolomics. Methods After rats were injected with L-levothyroxine sodium at a dosage of 35 μg · ( 100 g)-1 every day for 1 week, and then Liuwei dihuang pills( LDPs ) were treated at a dose of 4. 7 g · kg-1 for 3 consecutive weeks. The serum samples for rats were obtained after the treatment of LDPS and the metabolic profiles of serum were obtained with ultra performance liquid chromatography coupled with time-of-flight mass spectrometry ( UPLC-QTOF-MS ). Results The levels of oxaloacetic acid, Dihydroxyace-tone phosphate ( DHAP ), ethyl glucuronide, triglycer- ide, lysophospholipids and cholesterol were significantly increased in the model group as compared to the normal rats. Moreover, these levels could be regulated to be close to normal after the treatment of LDPS. Conclusions There is severe disturbance in the tricarbox-ylic acid cycle as well as sugar and lipid metabolism for hyperthyroidism rats. LDPS can exhibite obvious effects on the treatment of hyperthyroidism. Serum metabonomics analysis reveals the changes of metabolites traits during the treatment of LDPS, thus providing a supplementary evaluation method of LDPS in the treatment of hyperthyroidism.关键词
六味地黄丸/UPLC-QTOF-MS/代谢紊乱/三羧酸循环/甲亢阴虚/代谢组学/生物标志物Key words
Liuwei Dihuang pills/ UPLC-QTOF-MS/ metabolic disorders/ tricarboxylic acid cycle/ hyperthyroidism/ metabonomics/ biomarkers分类
医药卫生引用本文复制引用
王彬,沈岚,从文娟,朱云云,王强,冯怡..基于代谢组学的六味地黄丸干预大鼠甲亢模型的作用研究[J].中国药理学通报,2013,29(5):632-637,6.基金项目
国家科技重大专项(重大新药创制)(No 2009ZX09502-009),国家自然科学基金项目 （重大新药创制）
上海市教委重点学科自然基金(No J50302) （No J50302）
上海市科委2012年度"科技创新行动计划"(No 12401900402) （No 12401900402）
上海市教委创新项目(No 12ZZ124) （No 12ZZ124）
上海高校青年教师专项科研基金(No Szy11022) （No Szy11022）
上海市教委预算内科 （）
