中国药理学通报2013，Vol.29Issue(5)：664-669,6.DOI:10.3969/j.issn.1001-1978.2013.05.016
5-LO表达对铝盐致海马神经元损伤的影响
Effect of 5-LO expression on primarily cultured rat hippocampal neuron damage induced by aluminum
摘要
Abstract
Aim To investigate the effect of 5-LO expression on primarily cultured rat hippocampal neurons damage induced by aluminum. Methods Rat hippocampal neurons were primarily cultured for 7 days and treated by recombinant adenovirus expressing 5-LO ( Ad5-LO ) and Ad5-LO-RNAi. Primarily cultured neuron were divided into 7 groups, i. e. control group( 200 μmol · L-1 NaCl ), adenovirus-treated group ( MOI = 100 ), Ad5-LO-treated group( MOI = 100 ), Ad5-LO-RNAi-treated group( MOI = 100 ), adenovirus( MOI = 100) + aluminum ( 200 μmol · L-1 A1Cl3 )-treated group, Ad5-LO( MOI = 100 ) + aluminum( 200 μmol · L-1 A1Cl3 )-treated group and Ad5-LO-RNAi( MOI = 100) + aluminum ( 200 μmol · L-1 A1Cl3 )-treated group. The hippocampal neuron damage was determined by observation of the change of neuronal morphology and detection of SOD activity and MDA content in hippocampal neurons. The levels of 5-LO proteins and 5-LO mRNA expression were also measured. Results As compared with adenovirus-treated group, the neurons were injured remarkably, the optical density of MTT and activity of SOD were decreased, the leakage of LDH and content of MDA and expression of 5-LO mRNA and proteins were increased in adenovirus + aluminum-treated group. As compared with adenovirus + aluminum group, these changes were more obvious in Ad5-LO + aluminum-treated group. The treatment of Ad5-LO-RNAi could significantly blunt the changes by aluminum. Conclusions The overexpression of 5-LO increases the sensibility of hippocampal neuron to aluminum injury and the inhibition of 5-LO expression can protect the neuron from injury induced by aluminum.关键词
5-LO表达/铝/原代培养/海马神经元/腺病毒/神经元损伤/氧化应激Key words
5-LO expression/ aluminum/ primary culture/ hippocampal neuron/ neuron damage/ oxidative stress分类
医药卫生引用本文复制引用
王健峰,杨俊卿,黄砚,谢灵瑶,郭远新,雷文娟..5-LO表达对铝盐致海马神经元损伤的影响[J].中国药理学通报,2013,29(5):664-669,6.基金项目
国家自然科学基金资助项目(No 30672211,81070972) （No 30672211,81070972）
