中国药理学通报2013，Vol.29Issue(5)：708-714,7.DOI:10.3969/j.issn.1001-1978.2013.05.026
海地瓜硫酸软骨素对3T3-L1前脂肪细胞增殖和分化的影响
Effect of Acaudina Molpadioides chondroitin sulfate on the proliferation and differentiation of 3T3-L1 preadipocytes
摘要
Abstract
Aim To observe the effect of AM-CHS on the proliferation and differentiation of 3T3-L1 preadipocytes, and to investigate its possible mechanism. Methods After using differentiation cocktail to differentiate 3T3-L1 preadipocytes, the effects of AM-CHS on the proliferation activities of 3T3-L1 cells at differ-ent time were determined by MTT assay. Besides, the effect of AM-CHS on the differentiation of 3T3-L1 preadipocytes was carried out by oil red 0 staining and TG content determination. Expression levels of PPARγ,C/EBPα and SREBP-1c mRNA were measured by reverse transcription PCR( RT-PCR ). Results AM-CHS inhibited the proliferation of 3T3-L1 preadi-pocytes and mature adipocytes, and inhitited the differentiation of 3T3-L1 preadipocytes, in which the strongest inhibition appeared in the early state. RT-PCR results showed that AM-CHS down-regulated the expression of PPARγ, C/EBPα and SREBP-1c mRNA, which presented time-dose independence. Conclusion AM-CHS can markedly suppress the proliferation and differentiation of 3T3-L1 preadipocytes, and the underlying mechanisms may be related to down-regulating the expression of PPARγ,C/EBPα and SREBP-1c mRNA.关键词
海地瓜硫酸软骨素/3T3-L前脂肪细胞/分化/PPARγ/C/EBPα/SREBP-1cKey words
Acaudina Molpadioides chondroitin sul-fate/ 3T3-L1 preadipocytes/ differentiation/ PPARγ/ C/ EBPα/ SREBP-1c分类
医药卫生引用本文复制引用
龙腾腾,王静凤,常耀光,贺敏,武凤娟,王玉明,薛长湖..海地瓜硫酸软骨素对3T3-L1前脂肪细胞增殖和分化的影响[J].中国药理学通报,2013,29(5):708-714,7.基金项目
"十二五" 国家科技支撑计划(No 2012BAD33B07) （No 2012BAD33B07）
海洋公益性行业科研专项(No 201105029) （No 201105029）
长江学者和创新团队发展计划资助 （）
