植物研究2013，Vol.33Issue(2)：174-180,7.DOI:10.7525/j.issn.1673-5102.2013.02.009
AtZW1O蛋白亚细胞定位的初步研究
Preliminary Study on the Subcellular Localization of Arabidopsis thaliana AtZW10
摘要
Abstract
The bioinformatics analysis of AtZWIO protein was performed by using different approaches.The results showed that the Arabidopsis thaliana AtZWIO protein is much conserved during the evolution of high eukaryotes.In AtZWO protein,there is a similar conserved region to the centromere/kinetochore-binding protein ZW10.Moreover,the high similarity was found between AtZWO and the Drosophila DmZWIO protein.AtZWIO was considered to be a hydrophilic protein by hydrophobicity analysis,and no obvious transmembrane domains.Prediction results also indicated that AtZWIO is likely to be located in the nucleus and cytoplasm.In order to further determine the subcellular localization of AtZWIO,the full-length of AtZWIO gene was amplified by PCR using Arabidopsis cDNA as a template,and the binary vectors consisting of AtZWIO fused with the yellow (green) fluorescent protein (YFP/GFP) coding sequence were further constructed.The recombinant vectors were transformed into wild-type Arabidopsis protoplasts of mesophyll cells and Nicotiana benthamiana epidermal cells,respectively.Subsequently,the analyses on the distribution of the fusion proteins suggest that AtZWIO is an nucleoplasm positioning protein,and is mainly distributed in the nucleus,consistent with the conclusions of bioinformatics analysis.The molecular features and the subcellular localization of AtZWIO were analyzed in the present study,which might provide a basis for studying the function of AtZWIO gene in the future.关键词
AtZW10/生物信息学分析/载体/融合蛋白/亚细胞定位Key words
AtZWIO/bioinformatic analysis/vector/fusion proteins/subcellular localization分类
生物科学引用本文复制引用
陆玉建,刘恒,李玉玺,梁大伟,赵鹏,贾鹏飞,钮松召..AtZW1O蛋白亚细胞定位的初步研究[J].植物研究,2013,33(2):174-180,7.基金项目
滨州学院博士基金项目(2010Y08) （2010Y08）
山东省自然科学基金项目(ZR2012CL14) （ZR2012CL14）
