植物研究2013，Vol.33Issue(2)：186-190,5.DOI:10.7525/j.issn.1673-5102.2013.02.011
增强UV-B辐射对拟南芥叶肉细胞蛋白的影响
Effect of Enhanced UV-B Radiation on Arabidopsis Mesophyll Cell Protein
摘要
Abstract
Four-week-old wild-type Arabidopsis seedlings ( Columbia-O) was treated using different doses of UV-B radiation,the protein in the mesophyll cells was extracted using acetone precipitation and TCA-acetone,then Arabidopsis mesophyllcellular protein content and composition of the different intensity of UV-B radiation response were analyzed.The results showed that comparing two methods,the protein content of the TCA-acetone extract is relatively higher,which was more suitable for the analysis of enhanced UV-B radiation on Arabidopsis mesophyll cell protein; The changes of protein contents by two extraction methods showed the same trend,along with the increasing of UV-B radiation dose,the protein content increased first and then reduced,B2 group reached a maximum.In addition,the number of protein bands and expression has taken place significant change,the most obvious changes were also in the middle dose treatment group ( B2) ,both new bands and disappearance bands.This may be due to the Arabidopsis thaliana can activate some of its own resistance gene expressions and induce resistance protein by low doses of UV-B radiation,and thus resist the damage of UV-B; however,when subjected to high doses of UV-B radiation,damage their own protein synthesis pathway,and affect protein synthesis.关键词
UV-B辐射/拟南芥/蛋白Key words
UV-B radiation/Arabidopsis/protein分类
生物科学引用本文复制引用
魏小丽,郑娜,李晓阳,韩榕..增强UV-B辐射对拟南芥叶肉细胞蛋白的影响[J].植物研究,2013,33(2):186-190,5.基金项目
国家自然科学基金资助项目(30671061) （30671061）
山西省自然科学基金资助项目(20081101) （20081101）
