植物研究2013，Vol.33Issue(2)：191-196,6.DOI:10.7525/j.issn.1673-5102.2013.02.012
基因枪法获得无选择标记的1 Dx5基因表达的转基因小麦
Transformation and Expression of 1 Dx5 without Selectable Markers in Common Wheat Xindong No.26
摘要
Abstract
To improve the bread-making quality of wheat,the Xinjiang winter common wheat Xindong No.26 were transformed with high-molecular-weight glutenin subunits ( HWM-GS) gene 1 Dx5 that without selectable markers by particle bombardment.Transformed plants were screened by PCR,three transgenic plants were detected among 1 000 transformed plants,yielding a transformation efficiency of 0.3%.The compositions of HWM-GS were analyzed by SDS-PAGE.The HMW-GS gene 1Dx5 was expressed in some seeds of transgenic plants.The high molecular weight glutenin subunit gene 1 Dx5 was transformed into wheat XinDong No.26 and was expressed in some transgenic progenies.Our studies lay the foundations for the improvement of bread-making quality of wheat.关键词
小麦/选择标记/1 Dx5/基因枪Key words
wheat/selectable markers/1 Dx5/microprojectile分类
农业科技引用本文复制引用
王勇,覃建兵,范玲,祝长青..基因枪法获得无选择标记的1 Dx5基因表达的转基因小麦[J].植物研究,2013,33(2):191-196,6.基金项目
国家自然科学基金(30960044) （30960044）
本项目由新疆师范大学研究生创新基金项目资助(项目名称:小麦遗传转化体系建立及高分子量麦谷蛋白亚基基因1Dx5的基因枪转化 （）
项目编号:20121208) （）
