植物研究2013，Vol.33Issue(2)：232-237,6.DOI:10.7525/j.issn.1673-5102.2013.02.019
基于SSR标记构建葡萄种质资源分子身份证
Establishment of Molecular ID for Grape Germplasm Based on SSR Markers
摘要
Abstract
Using a total of 80 grape germplasms selected from national grape repository of Zhengzhou as the material,the methods of establishing the grape germplasms molecular ID were studied.Germplasms were distinguished by the selected SSR markers,and then coded the size of augmentation strip molecular based on the different germplasms.The results showed that after screening of the 62 primers,169 alleles were detected using 28 selected SSR markers located on each chromosome of grape,with a mean value of 6.0 alleles/locus.After alleles assignment,there were only 9 pairs of primers selected out for the construction of germplasms molecular ID with an average value of 8.9 germplasms/primers.The purpose of distinguishing the variety of grape was achieved by a minimum of primers.The results suggested SSR marker techniques can be used for establishing molecular identity of grape germplasm.关键词
葡萄/SSR/分子身份证Key words
grape ( Vitis)/SSR/molecular ID分类
农业科技引用本文复制引用
杜晶晶,刘国银,魏军亚,刘德兵,杨小振..基于SSR标记构建葡萄种质资源分子身份证[J].植物研究,2013,33(2):232-237,6.基金项目
海南大学青年基金(qnjj1188) （qnjj1188）
现代农业产业技术体系专项资金(CARS-30-yz-1) （CARS-30-yz-1）
农作物种质资源保护项目(NB2011-2130135-34) （NB2011-2130135-34）
