郑州大学学报（理学版）2013，Vol.45Issue(1)：90-94,5.DOI:10.3969/j.issn/1671-6841.2013.01.022
PCR-Tag荧光技术在检测鲍曼不动杆菌中的初步应用
Detecting Acinetobacter baumannii by PCR-Tag Fluorescence Technology
摘要
Abstract
It was important to find a method for analyzing PCR products by automatic analysis station in clinical labs. A total of 208 clinical samples of Acinetobacter baumannii were examined by traditional PCR-agarose gel electrophoresis and PCR-Tag fluorescence, respectively. The results showed that two methods presented similar results in the sensitivity without statistical significance. PCR-Tag method showed low fluorescent background and high signal-to-noise (S/N) ratio. The procedure of PCR-Tag fluorescence seemed less DNA contaminated. The technology had a potential to be used as an instant detection method in clinical labs. It was suitable for the automatic sample analyzers in clinical labs.关键词
PCR-Tag荧光/PCR/鲍曼不动杆菌Key words
PCR-Tag fluorescence/ PCR/ Acinetobacter baumannii分类
生物科学引用本文复制引用
张晓千,时文化,史团省,赵万千..PCR-Tag荧光技术在检测鲍曼不动杆菌中的初步应用[J].郑州大学学报（理学版）,2013,45(1):90-94,5.
