华北农学报2013，Vol.28Issue(1)：1-6,6.
西瓜防御素(ClPDF)基因挖掘及序列分析
Gene Mining and Sequence Analysis of Plant Defensins in Citrullus lanatus
摘要
Abstract
To clarify the structure and functional characterization of defensin genes in watermelon, this work a-dopted the bioinformatics methods to analyze the watermelon defensin gene family and sequence characteristics in detail based the fully sequenced watermelon genome. The results showed that a family of 7 watermelon defensin ( CIPDF) genes was discovered by genome sequence analysis in watermelon. Alignment of CIPDFs showed the presence of highly conserved eight cysteines,a glycine residue,a serine and an aromatic residue. Phylogenetic analysis revealed that CIPDFs belonged to Arabidopsis PDF2 gene cluster. CIPDFs proteins were found varies in structures. Secondary structure assay showed that random coil is the main composition,whereas the percent of extended strand and alpha helix has some differences. SignalP analysis result parallels with that of TMHMM .namely .except for other six genes, only one gene without signal peptide and one gene has the transmembrane segment. This work proved that defensin genes were multiple gene family in watermelon genome, and all the watermelon defensin genes contained the highly conserved domain but exhibited significant differences in structure characteristics among watermelon defenses, This work will provide foundation for the identification of biological functions of watermelon difensins.关键词
西瓜/防御素/基因进化/生物信息学Key words
Watermelon /Plant defensins/Gene evolution/Bioinformatics分类
农业科技引用本文复制引用
张曼,羊杏平,徐锦华,刘广,姚协丰,李苹芳..西瓜防御素(ClPDF)基因挖掘及序列分析[J].华北农学报,2013,28(1):1-6,6.基金项目
国家西甜瓜产业技术体系(CARS-NO.8) （CARS-NO.8）
江苏省农业科技自主创新基金项目(cx(11)1001) （cx(11）
江苏省农业科学院博士后基金(006046511105) （006046511105）
