华北农学报2013，Vol.28Issue(1)：62-66,5.
小麦返白系相关基因的克隆与表达
Cloning and Expression Analysis of Related Gene in Wheat(Triticum aestivum L.)Albinism Line
摘要
Abstract
In order to study the molecular mechanism of the " albino-after green" in wheat ( Triticum aestivum L. ) albinism line, we have designed the PCR primer with the LSC of Chloroplasts genome sequence in Chinese spring wheat. We have amplified the related gene segment in chinese spring wheat, albinism line and Aibian 1 and get the specific gene fragment WFC01 in albinism line. The total RNA has been extracted from leaves in Chinese spring wheat, Aibian 1 and albinism line. WFC01 was the probe for northern blot. The results show that the expression of gene WFC01 has been inhibition along with the leaves albinism in albinism line compared with albinism line and Chinese spring wheat. The gene WFC01 expression was back to normal with the leaves get green.关键词
小麦/返白系/白化/基因表达/Northern杂交Key words
Wheat/ Albinism line/ Albinism/ Clone/ Northern blot分类
生物科学引用本文复制引用
侯典云,胥华伟,杜光源,郭蔼光,徐虹..小麦返白系相关基因的克隆与表达[J].华北农学报,2013,28(1):62-66,5.基金项目
国家自然科学基金(30971769) （30971769）
河南省教育厅科学技术研究重点项目(12B180007) （12B180007）
河南科技大学人才科学研究基金(09001428) （09001428）
河南科技大学青年科学基金(2010QN0004) （2010QN0004）
河南科技大学实验开发基金(SY1011043) （SY1011043）
