华北农学报2013，Vol.28Issue(1)：70-75,6.
牛游离脂肪酸受体1基因研究
The Research of Bovine Free Fatty Acid Receptor-1 Gene
摘要
Abstract
Free fatty acid receptor l(GPR40)is a G-protein coupled receptor. When bound by free fatty acid,it can activate the intracellular signal pathway and regular the cell function. Many researches showed that the ffar-1 played an important role in pancreatic tissue and nervous system. But the mechanism how it works is not clear,especially in nervous system. Predicts and analysises the core promoter using bioinformatics methods. Takes the use of 5' RACE technology to amplify the 5'UTR of bovine ffar-1 mRNA. And constructs the eukaryotic expression vector. The core promoter of bovine ffar-1 gene is the type of TATA-less and Int-DPE promoter. Prolongs the mRNA of bovine ffar-1 gene forward by 726 bp. And the eukaryotic expression vector words well in bovine fibroblast cells. Studies the bovine ffar-1 gene in 3 aspects, the promoter sequence, mRNA 5' UTR, and eukaryotic expression and provides the theoretical and experimental basis for the research of the function and regulation of bovine ffar-1 gene.关键词
游离脂肪酸受体1/启动子/真核表达Key words
Free fatty acid receptor 1/ Promoter/ Eukaryotic expression分类
生物科学引用本文复制引用
武珅,浦亚斌,李向臣,关伟军,马月辉..牛游离脂肪酸受体1基因研究[J].华北农学报,2013,28(1):70-75,6.基金项目
转基因生物新品种培育科技重大专项(2011ZX08009-003-006 （）
2011ZX08012-002-06) （）
基本科研业务项目(2012ZL072) （2012ZL072）
国家家养动物种质资源平台项目(2012) （2012）
