华北农学报2013，Vol.28Issue(1)：76-81,6.
牦牛MSTN基因分子克隆及序列分析
Cloning and Sequence Analysis of Yak MSTN Gene
摘要
Abstract
The yak MSTN gene was amplified using particular primers,then cloned and sequenced. It was obtained by sequence splicing using molecular biology software. The results showed that yak MSTN gene ( GenBank Accession No: EU926670) had 3 exons and 2 introns. The size of CDS (GenBank Accession No: EU926671 ) sequence of the yak MSTN gene,encoding 375 amino acid,was 1 128 bp. The size of exon Ⅰ ,exon Ⅱ and exon Ⅲ was 373,374,374 bp, respectively; while the size of intron I and intron H was 1 843,2 024 bp, respectively. A single base conversion( C→T) located 417 base was found in Yak MSTN gene coding region compared to ordinary cattle, which did not cause changes in amino acids. The nucleotide sequence of the coding region and deduced amino acid sequences among different species were quitely conservative. The homology rates of nucleotide sequences of the coding region of MSTN gene between the yak and cattle,sheep,pig,human,dog,mouse,house,rabbit,chick and chimpanzee were 99. 9% ,96.4% ,94.6% ,92.2% ,91.8% ,91.8% ,92.8% ,95.3% ,84.4% ,84.9% .respectively, while the homology rates of amino acid sequences were 100% ,93.3% ,95.5% ,92.5% ,94. 1% ,93.3% ,94.9% , 94.4% ,88.0% and 94.4% .respectively. Biological software analysis showed that the theoretical molecular weight of Yak MSTN gene was 42. 6 kDa and PI was 6. 14. Leu was the highest(9. 9% ) .followed by Lys(7. 2% ). The secondary structure of Yak MSTN protein was mainly β-folding and it had characteristics of transmembrane protein structure. And the transmembrane domain located between AA6 and AA23. Moreover,it had a secretion signal peptide structure,MQKLQICVYIYLFMLIVA,which probably related well with TGF-β protein family.关键词
牦牛/MSTN基因/克隆/序列分析Key words
Yak/MSTN gene/Cloning/Sequence analysis分类
生物科学引用本文复制引用
梁春年,丁学智,包鹏甲,郭宪,裴杰,王宏博,褚敏,刘文博,吴晓云..牦牛MSTN基因分子克隆及序列分析[J].华北农学报,2013,28(1):76-81,6.基金项目
公益性行业科技专项经费(201003061 （）
201203008) （）
甘肃省牦牛繁育工程重点实验室(1106RTSA015) （1106RTSA015）
现代农业肉牛牦牛产业技术体系专项经费(CARS-38) （CARS-38）
国家科技支撑计划(2012BAD13B00) （2012BAD13B00）
