华北农学报2013，Vol.28Issue(1)：82-87,6.
杜梨S-腺苷甲硫氨酸脱羧酶基因的克隆与生物信息学分析
Cloning and Bioinformatics Analysis of S-adenosylmethionine Decarboxylase Gene in Pyrus betulaefolia Bge.
摘要
Abstract
In this study, S-adenosylmethionine decarboxylase, designated as PbSAMDC ( GenBank accession number; JX624260) ,was isolated from Pyrus betulaefolia Bge. by homologous cloning and RACE technology. The results showed that the full-length cDNA sequence of PbSAMDC is 1 683 bp and encodes 358 amino acids, molecular weight and isoelectric point of the protein is 39. 767 7 kDa and 4. 96 respectively. And the deduced amino acids homology analysis indicated that PbSAMDC has a high homology with MdSAMDCl (96% ) , and then the grape, sweet orange. In the same time, biological activity of PbSAMDC was discussed in the end. The research has an important theoretical and practical significance for developing stress regulation mechanism of SAMDC and stress-tolerance breeding of pear.关键词
梨/S-腺苷甲硫氨酸脱羧酶/生物信息学Key words
Pear/ S-adenosylmethionine decarboxylase/ Bioinformatics分类
生物科学引用本文复制引用
张梅,王然,马春晖,段艳欣,李鼎立..杜梨S-腺苷甲硫氨酸脱羧酶基因的克隆与生物信息学分析[J].华北农学报,2013,28(1):82-87,6.基金项目
山东省优秀中青年科学家科研奖励基金(BS2011NY007) （BS2011NY007）
现代农业产业技术体系建设专项资金资助(CARS-29) （CARS-29）
青岛农业大学高层次人才启动基金(630920) （630920）
