华北农学报2013，Vol.28Issue(1)：88-92,5.
生菜rbcS基因启动子序列的克隆与分析
Cloning and Sequence Analysis of the Lettuce rbcS Promoter
摘要
Abstract
The 1 046 bp 5' upstream regions of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit gene (rbcS) was amplified from lettuce genome DNA by PCR under the direction of the primers, which were designed based on the rbcS gene and the promoter sequence of other plants. Sequencing analysis indicated that the fragment contains core elements CAAT-box and TATA-box,as well as the elments sensitive to light,MeJA,abscisic acid,eth-ylene,and heat stress. Meristem expression regulatory element was also found in the cloned rbcS promoter sequence. Furthermore, the rbcS promoter sequence of lettuce is similar to the rbcS promoters isolated from other plants of As-teraceae, Solanaceae or Fabaceae. This work provides strong support for high-level expression of recombinant proteins in lettuce.关键词
生菜/1,5-二磷酸核酮糖羧化/加氧酶/启动子/序列分析Key words
Lettuce/ Ribulose-1 , 5-bisphosphate carboxylase/oxygenase small subunit/ Promoter/ Sequence analysis分类
生物科学引用本文复制引用
董浩,赵轶君,李红民..生菜rbcS基因启动子序列的克隆与分析[J].华北农学报,2013,28(1):88-92,5.基金项目
陕西省教育厅2009年度重点实验室科研计划项目(09JS079) （09JS079）
陕西省科技厅2011年度社会发展攻关项目(2011K12-61) （2011K12-61）
陕西省教育厅重点实验室重点科研计划项目(11JS085) （11JS085）
