华北农学报2013，Vol.28Issue(1)：128-134,7.
苏钟猪FTO基因的mRNA表达、克隆测序及其生物信息学分析
The mRNA Expression, Cloning and Sequencing of FTO gene and Bioinformatics Analysis in Suzhong Pig
摘要
Abstract
Fat mass and obesity associated gene (FTO) plays an important role in the control of appetite and energy expenditure. In order to understand the structure and function of porcine FTO gene, the mRNA expression profile of porcine FTO in five tissues ( lung, liver, heart, backfat and longissimus dorsi muscle) were examined in Suzhong pigs by RT-PCR. Besides, the coding sequences (CDS) of FTO were cloned and sequenced in Suzhong pigs, molecular structure and homology among different species of FTO were analyzed by bioinformatics methods. The results showed that mRNA expression intensity of FTO in different tissues tended to be backfat > heart > lung > liver > longissimus dorsi muscle. Among these tissues, FTO expression in backfat was significantly higher than that in any other tissue (P < 0. 05 ). There were eight SNPs detected in coding sequences (CDS) of FTO in Suzhong pigs,including G52A , C523T, G628A, A655G, A810G, A886G, G1002A and A1344G. Bioinformatic analysis revealed that porcine FTO gene encode a protein of 505 amino acid, and its theoretical isoelectric point was 5.23. FTO protein was a hydrophilic and non-secreted protein without a signal peptide. In addition, Suzhong pig was closed to cattle, sheep and dog in phylogenetic tree. These findings suggest that the mRNA expression of FTO may have certain influence in regulating fat deposition in some tissues of Suzhong pigs, such as subcutaneous fat, heart and muscle,etc,and these abundant SNPs in coding sequence of FTO could become important molecular markers for improving meat quality in Suzhong pigs.关键词
苏钟猪/脂肪肥胖相关基因/克隆测序/遗传变异/生物信息学Key words
Suzhong pig/Fat mass and obesity associated gene/Cloning and sequencing/Genetic variation/Bioinformatics分类
生物科学引用本文复制引用
付言峰,李兰,王学敏,李碧侠,方晓敏,赵芳,任守文..苏钟猪FTO基因的mRNA表达、克隆测序及其生物信息学分析[J].华北农学报,2013,28(1):128-134,7.基金项目
国家自然科学基金(31201767) （31201767）
江苏省博士后科研资助计划经费 （）
江苏省农业科学院博士后基金(No.026056511104) （No.026056511104）
国家生猪现代产业技术体系南京综合试验站(nycytx-009) （nycytx-009）
