华北农学报2013，Vol.28Issue(1)：227-233,7.
芝麻太空诱变效应及AFLP标记检测
Effects of Space-induced Mutation and Analysis of Polymorphsim by AFLP Molecular Marker in Sesame
摘要
Abstract
The seeds of five sesame varieties ( Yuzhi No. 11, Japan black sesame, Zhengzhi D15,Zhengzhi 05N01 and Zhengzhi 06msl98 were carried to space for inducing mutation by Shijian 8 satellite. The agronomic trait and resistant variants of SP3 and SP4 were observed, and the genetic diversity of mutation descendants of Yuzhi NO. 11 and Japan black sesame was analyzed using 16 pair of AFLP markers. The results showed that the variants of leaf .capsule, flower color, childbearing period, plant height, first capsule height, yellow apex length, capsule number per plant, 1000-seed weight,yield and disease resistance were observed in SP3 and SP4 of different sesame varieties, but the variation frequency and variation type were significantly different among different sesame varieties. The agronomic traits showed more negative mutations than positive mutations, but resistance traits had more positive mutations. Compared to CK( without space mutation) ,the plant height,first capsule height,yellow apex length,capsule number per plant, 1000-seed weight and yield increased by 53 cm,77 cm,16 cm, 122/plant,0. 53 g,478. 54 kg/ha for positive mutation respectively,and decreased by 36 cm,30 cm, 19 cm, 197/plant,0. 64 g,l 013.81 kg/ha for negative mutation respectively. AFLP analysis indicated that the genetic distance of Yuzhi No. 11 was 0. 128 8 -0. 488 5 ,of which 72% was from 0. 12 to 0. 25 and the average genetic distance was 0. 25. The genetic distance of Japan black sesame was from 0. 289 7 to 1. 301 3 , of which 37. 5 % was from 0. 178 3 to 0. 499 6 and the average was 0. 687 6. It was concluded that the space-induced mutation had no direction and great randomness, and the sensitivity was different in sesame varieties.关键词
芝麻/太空诱变/AFLP标记/多态性分析Key words
Sesame/Space-induced/AFLP molecular marker/ Analysis of polymorphsim分类
农业科技引用本文复制引用
高桐梅,卫双玲,李春明,张海洋,李丰,贺松涛..芝麻太空诱变效应及AFLP标记检测[J].华北农学报,2013,28(1):227-233,7.基金项目
现代农业产业技术体系建设项目专项资金资助(CARS-15) （CARS-15）
河南省省院科技合作项目(112106000023) （112106000023）
