实验动物与比较医学2013，Vol.33Issue(2)：106-111,6.DOI:10.3969/j.issn.1674-5817.2013.02.005
慢性缺O2高CO2性大鼠小脑水肿及姜黄素的保护作用
Chronic Hypoxia-high Carbon Dioxide-induced Cerebellum Edema and Protective Effect of Curcumin in Rat
摘要
Abstract
Objective To explore expression of AQP4 and edema in hypoxia- high carbon dioxide rat cerebellum edema model and the protective effects of curcumin. Methods Fifty-five adult male SD rats were randomly divided into 3 groups: the control group, the experimental group and curcumin treated group. The experimental group and curcumin group were housed in atmospheric hypoxia- high carbon dioxide environment to induce hypoxia-high carbon dioxide brain edema model. The difference of the hypoxia-high carbon dioxide of rat cerebellar cells edema degree were observed under electron microscopic after HE staining and immunohistochemistry. The expression level of AQP4mRNA in the experimental group and the control group were detected using Real time fluorescence PCR. The results showed that cerebellar cells edema was more significant and AQP4 gene expression was relatively higher in the experimental group rat than curcumin group. Conclusion Rat cerebellar edema can be developed in hypoxia-high carbon dioxide environment, and it may be related to the expression of AQP4 gene, Curcumin has protective effect on such edema.关键词
慢性缺O2高CO2/AQP4/姜黄素/实时荧光PCRKey words
Hypoxia-high carbon dioxide/ AQP4/ Real time fluorescence PCR/ Curcumin分类
生物科学引用本文复制引用
毛香岚,赵翔,李泽杰,蔡鸿勇,张涛,叶光华,喻林升..慢性缺O2高CO2性大鼠小脑水肿及姜黄素的保护作用[J].实验动物与比较医学,2013,33(2):106-111,6.基金项目
温州医学院学生科研立项重点资助课题(wyx201201005) （wyx201201005）
