实验动物与比较医学2013，Vol.33Issue(2)：121-125,5.DOI:10.3969/j.issn.1674-5817.2013.02.008
低氧对大鼠海马神经干细胞增殖的影响
Effects of Hypoxia on Proliferation of Hippocampal Neural Stem Cells in Rat
摘要
Abstract
Objective To observe the effects of hypoxia on proliferation of rat's hippocampal neural stem cells. Methods The hippocampal neural stem cells from newborn SD rats were cultured in the concentration of 1%, 4%, and 20% oxygen respectively, and the diameter and number of neurospheres were observed under the low oxygen. Results (1) Effects of hypoxia on proliferation efficiency. The number of neurospheres of 1% group was significantly lower than control group, (P<0.05). The number of neurospheres of 4% group was higher than control group, but no significantly difference. (2) Effects of hypoxia on proliferation rate. The diameter of 1% group was significantly shorter than control group at 24 h, 36 h and 48h,and reached the extremely significant levelat 48 h (P<0.01). The diameter of 4% group was longer than control group in 24 h, 36 h and 48 h. There was a significant difference at 36h and an extremely significant difference at48 h. (P<0.01). Conclusion 4% oxygen concentration can not only improve proliferation efficiency of rat's hippocampal neural stem cells, but also improve its proliferation rate. 1% oxygen concentration can not only inhibit proliferation efficiency of rat's hippocampal neural stem cells, but also inhibit its proliferation rate. This suggests us that moderate hypoxia can improve the proliferation of rat's hippocampal neural stem cells, while server hypoxia inhibits its proliferation.关键词
低氧/神经干细胞/增殖/海马Key words
Hypoxia/ Neural stem cells/ Proliferation/ Hippocampus分类
社会科学引用本文复制引用
王世强,娄淑杰..低氧对大鼠海马神经干细胞增殖的影响[J].实验动物与比较医学,2013,33(2):121-125,5.基金项目
国家自然科学基金(No.30870349)和运动健身科技省部共建教育部重点实验室(上海体育学院)共同资助 （No.30870349）
