实验动物与比较医学2013，Vol.33Issue(2)：130-134,5.DOI:10.3969/j.issn.1674-5817.2013.02.010
小型猪腹壁拉链装置的细胞毒性试验及排异反应观测
The Cytotoxicity Test and Rejection Observation of Abdominal Zipper for Miniature Pigs
摘要
Abstract
Objective To assess the safety of abdominal zippers of miniature pig by cytotoxicity test and rejection observation. Methods The safety of abdominal zippers of miniature pig were evaluated by CCK-8 cytotoxicity test. In the test of rejection observation, nine miniature pigs were randomly divided into three groups: control group(C group), surgical procedure group(S group) and the animal model of miniature pig with abdominal zipper group(M group), and IL-1, IL-6, IL-10 and TNF-a in blood serum were detected before surgery (0 d), and during the day of 1d, 3 d, 7 d, 14 d and 21 d after surgery. Results The cytotoxicity grade was 0 or Ⅰ level, and the level of IL-1, IL-6, IL-10 and TNF-a in S group and M group increased significantly at 1d and 3 d after surgery, but there was no difference between S group, M group and C group at 7 d, 14 d and 21 d after surgery. Conclusions The abdominal zipper for miniature pigs, which satisfies the national safety standard of biological materials, has qualified cytotoxicity and excellent biosafety.关键词
小型猪/生物拉链/细胞毒性试验/排异反应Key words
Miniature pig/ Bio-zipper/ Cytotoxicity test/ Rejection分类
医药卫生引用本文复制引用
余琛琳,汤球,刘志学,马雷,徐晨,蔡丽萍,崔淑芳..小型猪腹壁拉链装置的细胞毒性试验及排异反应观测[J].实验动物与比较医学,2013,33(2):130-134,5.基金项目
上海市科技发展基金项目(08140901400,09140903100) （08140901400,09140903100）
