现代检验医学杂志2013，Vol.28Issue(2)：84-86,89,4.DOI:10.3969/j.issn.1671-7414.2013.02.025
鲍曼不动杆菌碳青霉烯酶相关耐药基因的初步分析
Primary Analysis of Carbapenemase-Encoding Drug ResistanceCorrelated Gene of Acinetobacter Baumannii
尹秀杉 1姜伟2
作者信息
- 1. 南充市中心医院检验科,四川南充,637000
- 2. 四川省医学科学院四川省人民医院检验科,成都,610072
- 折叠
摘要
Abstract
Objective To investigate antibiotic resistance and carbapenemase genotype in Acinetobacter baumannii.Methods 71 strains of Acinetobacter baumannii resistant to carbapenem were collected from sputum of inpatients in ICUs of Sichuan Provincial People's Hospital from July 2010 to December 2011.Their minimal inhibitory concentrations (MICs) were done by Kribry-Bauer methods and microbroth dilution.Using the modified Hodge Test detection carbapenemase,The genes were amplified by polymerase chain reaction (PCR),and the PCR products were sequenced.Results 71 strains showed multi-drug resistance.There were 70 strains with the carbapenemase.The OXA-23 gene was identified in 69 of the 71 strains.The OXA-24,KPC,SHV and IMP gene were not identified.The sequenced results were absolutely homology with blaOXA-23 gene.Conclusion BlaOXA-23 gene is the major gene of carbapenemase genotype in Acinetobacter baumannii,and the antibiotic resistance was high.关键词
鲍曼不动杆菌/碳青霉烯酶/基因Key words
acinetobacter baumannii/ carbapenemase encoding/ gene分类
医药卫生引用本文复制引用
尹秀杉,姜伟..鲍曼不动杆菌碳青霉烯酶相关耐药基因的初步分析[J].现代检验医学杂志,2013,28(2):84-86,89,4.
