西安交通大学学报（医学版）2013，Vol.34Issue(3)：331-335,340,6.
小鼠急性光损伤视网膜组织中c-Fos、Caspase-1蛋白的表达及依达拉奉的干预作用
C-Fos and Caspase-1 protein expressions in mice with acute retinal photodamage and the interventional effect of edaravone
耿园园 1张小玲 1黎彦宏 1鱼喆 1张富军1
作者信息
- 1. 西安交通大学医学院第一附属医院眼科,陕西西安710061
- 折叠
摘要
Abstract
Objective To explore the protective effect of edaravone on acute retinal photodamage (RP) in mice. Methods Totally 42 adult male BALB/c mice were randomly divided into normal group (n = 6) and experimental group ( n = 36). The latter was subdivided into light group (group L, n = 18), edaravone group (group E, n = 18), and saline group (group NS, n = 18). Mice in experimental group were exposed to (8 000 ± 300) lux white fluorescent continuously for 4 hours to establish retinal RP model. Mice were killed at 0, 24, 72 h of dark repair after light exposure. HE staining of retinal tissue sections was used to observe morphologic changes of the retina and to measure thickness of retinal outer nuclear layer (ONL). Immunohistochemical staining was used to test c-Fos and Caspases-1 expressions. All results were combined to evaluate the protective effect of edaravone on RP. Results ONL became thinner in experimental group with longer dark repair after light exposure than that in normal group. At the same time point, compared to group L and group NS, the thickness of ONL was significantly preserved in group E (P<0. 05). At 0 h dark repair, expressions of c-Fos and Caspase-1 protein could be detected in ONL in each experimental group. Each peak of them was at 0 h and 24 h dark repair, respectively. At the same time point, expressions of both c-Fos and Caspase-1 protein were decreased significantly in group E compared to group L and group NS (P<0.05). Conclusion RP can decrease the thickness of ONL and upregulate the expressions of c-Fos and Caspase-1 protein. Edaravone can protect indirectly the retina from RP by inhibiting c-Fos and Caspase-1 expressions.关键词
依达拉奉/视网膜光损伤/细胞凋亡/c-Fos/Caspase-1Key words
edaravone/ retinal photodamage/ apoptosis/ c-Fos/ Caspase-1分类
医药卫生引用本文复制引用
耿园园,张小玲,黎彦宏,鱼喆,张富军..小鼠急性光损伤视网膜组织中c-Fos、Caspase-1蛋白的表达及依达拉奉的干预作用[J].西安交通大学学报（医学版）,2013,34(3):331-335,340,6.
