西安交通大学学报（医学版）2013，Vol.34Issue(3)：341-345,5.
尿毒症大鼠肠道菌群失调及其与微炎症状态的相关性
Relationship between microbiome dysbiosis of intestinal flora and microinflammation in experimental uremic rats
摘要
Abstract
Objective To investigate microbiome dysbiosis of intestinal flora in experimental uremic rats and its correlation with microinflammation. Methods Healthy male SD rats were randomly divided into uremic group and sham group. Uremic model was established in uremic group by 5/6 nephrectomy, while sham group underwent nephrocapsulotomy. All rats were sacrificed at the end of week 10. Serum creatinine, urea nitrogen, IL-6 and CRP were examined. Histopathological changes of rat kidney were observed by HE and Masson staining. The numbers of Bifidobacterium, Lactobacillus and E. coli in the intestinal tract (jejunum, ileum and colon) were quantitatively determined by real-time fluorescent quantitative PCR. Results Serum IL-6 and CRP were significantly increased in uremic group (P<0.05). Compared with sham group, the number of E.coli in the jejunum increased significantly (P<0.05) while that of Bifidobacterium and Lactobaeillus in the ileum decreased (P<0,05). The number of Bifidobacterium and Lactobaeillus decreased in the colon whereas that of E. coli increased (P<0.05). Correlation analysis showed that the number of ￡. coli in the colon had a positive correlation with IL-6 in uremic group. Conclusion Microbiome dysbiosis of intestinal flora occurs in uremic rats and correlates with microinflammation. The former one may be one of the causes of the latter.关键词
尿毒症/微炎症状态/菌群失调/肠道/白细胞介素-6/C-反应蛋白/肌酐/尿素氮Key words
uremia/ microinflammation/ microbiome dysbiosis/ gut/ IL-6/ CRP/ Scr/ Bun分类
医药卫生引用本文复制引用
魏萌,蒋红利,王志刚,王旭珍,王萌..尿毒症大鼠肠道菌群失调及其与微炎症状态的相关性[J].西安交通大学学报（医学版）,2013,34(3):341-345,5.基金项目
国家自然科学基金资助项目(No.30872457/H0511) （No.30872457/H0511）
