新医学2013，Vol.44Issue(1)：68-72,5.DOI:10.3969/g.issn.0253-9802.2013.01.021
中华眼镜蛇毒对K562/ADM和难治性急性髓性白血病细胞作用研究
Effect of naja naja actra venom on K562/ADM and refractory acute myeloid leukemia cells
摘要
Abstract
Objective: To investigate the effects of naja naja atra venom (NNAV) on human erythroleukemia / adriamycin cells ( K562/ADM cells) and primary culture cells of early onset refractory acute myelogenous leukemia. Methods: Ten cases of the cells mentionedwas studied. After having being treated with different concentrations of NNAV, cell proliferations were all oberved with the method of thiazolyl blue tetrazolium bromide (MTT) . Expressions of Bcl-2, the apoptosis-related proteins was studied by the immunocytochemistry assay. Besides, flow cytometry was used to measure their apoptosis rate and effects of NNAV on cell cycle, and Western blot was for caspase-3 expression. Furthermore, we detected cyclin D expressions of the primary culture cells of early onset refractory acute myelogenous leukemia alone, and above methods were used to find difference in the cyclin-D-positive expression group and the negative group. Results: After different concentrations of NNAV treated, both of the K562/ADM cells and primary culture cells of early onset refractory acute myelogenous leukemia resulted in a failed Bcl-2 and a raised caspase-3 expression, as well as cell proliferations obviously suppressed, and they were found to be time and dose-dependent. NNAV with concentration of 0. 8 mg/L and 1. 0 mg/L could made cell cycle of the primary culture cells of early onset refractory acute myelogenous leukemia arrest in the G0/G1 phase, while the cell contents decreased at the G2 / M phase. For the primary culture cells of early onset refractory acute myelogenous leukemia alone, we observed that 7 of 10 were with CyclinD positive expression, the other 3 were with negative results. However, it was of no significant difference in the cyclin-D-positive expression group and the negative group. Conclusion: This vitro study revealed that NNAV significantly inhibited cell proliferations of both K562/ADM cells and primary culture cells of early onset refractory acute myelogenous leukemia, and it also helped to regulate the cell cycle arrest in the G0/G1 phase, reduced the cell content in the G2 / M phase. Our experiment suggested that NNAV possibly inhibited the cell proliferations of primary culture cells of early onset refractory acute myelogenous leukemia through the intervention of Bcl-2 and caspase-3 expression and induced them apoptosis.关键词
中华眼镜蛇毒/白血病/K562/ADM细胞/细胞周期蛋白DKey words
Naja naja actra venom/ Leukemia/ K562/ADM cells/ Cyclin-D引用本文复制引用
吴穗晶,杜欣,沈伟哉,李扬秋,凌伟,翁建宇,郭荣,钟立业,林伟..中华眼镜蛇毒对K562/ADM和难治性急性髓性白血病细胞作用研究[J].新医学,2013,44(1):68-72,5.基金项目
广东省科技厅项目(2009B060700060),广东省医学科学技术研究基金(A2009029) （2009B060700060）
