中国蔬菜Issue(18)：160-165,6.
等渗Ca(NO3)2和NaCl胁迫对黄瓜叶片叶绿体超微结构及光合特性的影响
Effects of Iso-osmotic Ca (NO3)2 and NaCl Stress on Chloroplast Ultra-structure and Photosynthesis in Cucumber leaves
摘要
Abstract
Taking nutrient solution as culture method, and salt sensitive cucumber ( Cucumis sativus L. ) cultivar ' Jinchun No. 2' as material, this experiment studies the effects of iso-osmotic Ca (NO3)2 and NaCl stress on cucumber seedlings' chloroplast ultra-structure, chlorophyll content, photosynthetic characteristics and chlorophyll fluorescence parameters. The results indicated that the chlorophyll content was decreased by both salt stress, and the decreasing degree of NaCl stress was bigger than that of Ca (NO3)2 stress. The ultra-structural analysis showed that under Ca (NO3)2 stress, most of the chloroplasts were out of the cell wall and the numbers of grana thylakoid were decreased; whereas under NaCl stress the structure of chloroplast displayed deformation, and the grana lamellae became inflation, the hungry particles increased significantly. The net photosynthesis rate ( Pn ), stomatal conductance ( Gs ), PS Ⅱ photochemical quantum yield ( Fv/Fm), PS Ⅱ actual photochemical efficiency ( ΦPSⅡ ) and photochemical quenching ( qP ) were all decreased by salt stress. However, the decreasing degree of Ca( NO3)2 stress was less than that of NaCl stress. Intercellular CO2 concentration( Ci) was decreased by Ca( NO3)2 stress, while was increased by NaCl stress. Thus, we concluded that the stomatal limitation was a dominate factor causing Pn decrease under Ca(NO3)2 stress and non-stomatal factor was a dominate factor causing Pn reduction under NaCl stress.关键词
黄瓜/盐胁迫/光合作用/叶绿素荧光/叶绿体超微结构Key words
Cucumber/ Salt stress / Photosynthesis / Chlorophyll fluorescence / Chloroplast ultra-structure分类
农业科技引用本文复制引用
童辉,张振兴,李斌,王军伟,郭世荣..等渗Ca(NO3)2和NaCl胁迫对黄瓜叶片叶绿体超微结构及光合特性的影响[J].中国蔬菜,2012,(18):160-165,6.基金项目
国家重点基础研究发展计划项目(2009CB119000),国家自然科学基金项目(31272209),现代农业产业技术体系建设专项资金项目(CARS-25-C-03),江苏省农业三新工程资助项目[SX(2011)289] （2009CB119000）
