中国兽医科学2013，Vol.43Issue(4)：339-344,6.
甘肃省及周边地区奶牛乳腺炎致病大肠杆菌的分离鉴定及其种系分群的研究
Identification and phylogenetic analysis of Escherichia coli isolated from dairy cows with clinical or subclinical mastitis in Gansu and surrounding areas
摘要
Abstract
Escherichia coli were isolated and characterized by biochemical analysis and its housekeeping sequence. Sequence profile gained by ERIOPCR and RAPD method were statistically analysed with cluster analysis in SPSS 17. 0,to evaluate the genetic similarity of the isolated E. coli from different areas. In result, 13 strains of E. coli were isolated from the samples. According to the gel maps and phyletic evolution analysis,group A was 61. 54%,group D was 44. 4%,group B2 was 7. 69%. And the 13 strains of E. coli would also be categoried into three groups by cluster analysis, 7 strains in type I (53. 85%), 4 strains in type II (30. 77%) and 2 strains in type HI (15. 38). Eigthy percent strains isolated from Qinghai belongs to type I , while 71. 43% strains from other three areas belongs to type II and type HI. When using the RAPD grouping system,all the 13 strains are grouped into two groups, type I 7 stains(53. 85%) and type II 6 strains(46. 15%),and 80% strains isolated from Qinghai belongs to type I , while other 83. 3% isolates from other three places belongs to type II . Genetic similarity showed that the E. coli isolated from Qinghai, are genetically,to some degree,different from the E. coli isolated from other three places located in southwest of Lanzhou.关键词
奶牛乳腺炎/大肠杆菌/种系分类群Key words
dairy cow mastitis/Escherichia coli/phylogenetic group分类
农业科技引用本文复制引用
张连梅,赵兴绪,张勇,张海容,胡俊杰,武小虎,徐燕霞,胡少辉,周长卿..甘肃省及周边地区奶牛乳腺炎致病大肠杆菌的分离鉴定及其种系分群的研究[J].中国兽医科学,2013,43(4):339-344,6.基金项目
"十二五"农村领域国家科技计划项目(2011AA10A210-6) （2011AA10A210-6）
兰州市科技局科技三项(033143) （033143）
