伪狂犬病病毒TaqMan荧光定量PCR方法的建立及应用OA北大核心CSCDCSTPCD
Development of TaqMan quantitative real-time PCR assay for detection of pseudorabies virus
为了进行猪伪狂犬病病毒的早期诊断,根据gE基因的保守序列设计了1对引物,构建阳性重组质粒,建立TaqMan荧光定量PCR检测方法.结果显示,该方法特异性强,对Bartha-K61弱毒疫苗株(gE-株)及其他病毒不发生交叉反应；敏感性高,最低检测下限为10 copies/μL,比常规PCR高100倍；重复性好,批内、批间重复试验的变异系数均小于2％.应用建立的方法与常规PCR分别对153份临床样品进行检测,检出率分别为67％、60％,二者的符合率是…查看全部>>
A pair of primers and TaqMan probes were designed based on the pseudorabies virus gE sequence, and then the real-time PCR assay was established for the detection of pseudorabies virus. The results indicated that the real-time PCR was highly specific,and there were no cross-reaction with the Bartha-K61(gE attenuated strain) and the related pathogens for the other swine diseases. The detection limit of the assay was 1 × 101 copies/μL,which was 100 fold higher …查看全部>>
徐玉;王晓玲;毕可东
青岛农业大学动物科技学院,山东青岛 266109烟台市动物疫病预防与控制中心,山东烟台264003青岛农业大学动物科技学院,山东青岛 266109
农业科技
猪伪狂犬病病毒TaqMan荧光定量PCR检测
porcinepseudorabies virusfluorescent quantitative PCRjdetection
《中国兽医科学》 2013 (4)
396-400,5
中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室基本业务费(SKLVBP201201)
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